Project 2 explores the biocontrol of bacteria in food by the application of phages directly onto the surface of food. A cocktail preparation of phages that can be applied in a spray format will be characterized, tested for efficacy, and compared against the leading agent for biocontrol of food-borne pathogens.
University of Santo Tomas
PCHRD-GIA
DOST Philippine Council for Health Research and Development
The general objective of the project is to develop a phage product to be used as biocontrol agent for foodborne pathogens
Specifically, it aims the following:
1. To isolate bacteriophages capable of lysing Salmonella enterica, and Listeria monocytogenes;
2. To characterize the isolated bacteriophages using plaque morphology, Transmission Electron Microscopy, Host range, Effect of pH and Temperature;
3. To determine the efficiency of the isolated bacteriophages as cocktail preparations in reducing the number of Salmonella and Listeria in selected food models at two temperatures (4 and 25 °C);
4. To screen isolated phages as candidates for therapeutic use in a cocktail preparation and compare their activity to the leading agent for biocontrol of food-borne pathogens; and
5. To develop a phage product containing a cocktail of phages that can be applied by the spray type method for application in raw and/or processed meat.
1. At least one (1) publication in an international journal
2. Presentation of results to national and international conferences
3. One (1) patent application for the phage product
4. Product Phage cocktail product that is applicable for use in different food samples like RTEs (fruits and vegetables) and dairy products
5. Mentored one (1) MSc in Microbiology or Biology student
6. Research linkage with Intralytix Incorporated, Maryland,USA
7. Partnership with technology adoptors: Gourmet Farms, Inc. (Silang, Cavite), and Magnolia, Inc. (Gen. Trias, Cavite; Sta. Rosa, Laguna)
At least one (1) publication in an international journal
Presentation of results to national and international conferences
One (1) patent application for the phage product
Product Phage cocktail product that is applicable for use in different food samples like RTEs (fruits and vegetables) and dairy products
Mentored one (1) MSc in Microbiology or Biology student
Research linkage with Intralytix Incorporated, Maryland,USA
Partnership with technology adoptors: Gourmet Farms, Inc. (Silang, Cavite), and Magnolia, Inc. (Gen. Trias, Cavite; Sta. Rosa, Laguna)