The project is focused on the development of an electrochemical biosensor array by immobilizing bacteriophages on an electrode surface and conforming the binding ability of the target bacteria and the bioreceptor by detecting a change in the change in impedance due to a biological event. Electrochemical biosensors using the impedance technique have been proven to be a promising method for detection of food-borne pathogenic bacteria due to their portability, rapidity, sensitivity, and capability of on-the-spot detection.
University of Santo Tomas
PCHRD-GIA
DOST Philippine Council for Health Research and Development
The project generally aims to develop an electrochemical impedance biosensor array using isolated as bioreceptors to detect selectively Salmonella spp. and Listeria monocytogenes.
Specifically, the project aims the following:
1. To isolate, propagate, and enrich bacteriophages that are lytic for Salmonella spp. and Listeria monocytogenes from environmental samples;
2. To characterize the isolated bacteriophages using Transmission Electron Microscopy (TEM), plaque morphology, host range studies, and studies on effect of pH and temperature;
3. To fabricate and characterize screen-printed carbon electrode arrays covalently modified with high density of bacteriophages against Salmonella spp. and Listeria monocytogenes using surface characterization techniques;
4. To optimize the different immobilization and measurement parameters based on the maximal charge transfer resistance (Rct) value obtained from the EIS instrument;
5. To evaluate the analytical performance of the biosensor array in detecting Salmonella spp. and Listeria monocytogenes; and
6. To apply the biosensor array to buffer extracts from raw and processed meat samples using standard addition method.
1. At least (1) publication in an international journal
2. Presentation of results to national and international conferences
3. One (1) patent application for the biosensor array
4. A portable biosensor device for the real-time screening of bacterial pathogens in meat
5. Mentored one (1) MSc in Chemistry student
6. Partnership with technology adaptors: Poultry and fresh meat (Magnolia, Monterey) and processed meats (Purefoods) divisions of San Miguel Pure Foods Company, Inc. (SMPFCI)
1. At least (1) publication in an international journal
2. Presentation of results to national and international conferences
One (1) patent application for the biosensor array
Portable biosensor device for the real-time screening of bacterial pathogens in meat
Mentored one (1) MSc in Chemistry student
Partnership with technology adaptors: Poultry and fresh meat (Magnolia, Monterey) and processed meats (Purefoods) divisions of San Miguel Pure Foods Company, Inc. (SMPFCI)